Part:BBa_K4241802

CvNDI1_CeB_TRAmy3D

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 1506
  Illegal PstI site found at 55
  Illegal PstI site found at 404
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 1506
  Illegal PstI site found at 55
  Illegal PstI site found at 404
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 1506
  Illegal PstI site found at 55
  Illegal PstI site found at 404
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 1506
  Illegal PstI site found at 55
  Illegal PstI site found at 404
  Illegal NgoMIV site found at 264
  Illegal NgoMIV site found at 1086
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 849
  

Overview

This composite part is an inducible high-expression system to produce Cecropin B, an antimicrobial peptide in microalgae. The expression of this part is triggered by nitrogen starvation. This part is based upon research from the following publication: https://www.nature.com/articles/s41598-020-69620-9

Usage and Biology

This part is used for the inducible expression of Cecropin B in a high-expression manner in microalgae. CvNDI1 is a strong promoter induced via nitrogen starvation. This promoter is derived from the urea carboxylase, and can be used to increase expression up to 20-fold as compared to wild-type expression.

Parts that comprise this composite component

Nitrogen Starvation Inducible promoter 1 (Microalgae) - Part:BBa_K4241790
CvNDI_UTR - BBa_K4241799
Cecropin B Q53 - Part:BBa_K4241793
TRAmy3D_UTR - BBa_K4241800
TRAmy3D - BBa_K4241794

References

Shin, J.-H., Choi, J., Jeon, J., Kumar, M., Lee, J., Jeong, W.-J., & Kim, S.-R. (2020). The establishment of new protein expression system using n starvation inducible promoters in chlorella. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-69620-9